Description: Gel filtration chromatography is used to separate large molecules on the basis of size. Two columns are run simultaneously. The first column contains Sephadex G-75, which separates blue dextran and hemoglobin. The second column contain Sephadex G-10, which separates hemoglobin and riboflavin. Because there is a difference in the two packing materials, the hemoglobin molecule runs very differently in the two columns.

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Source: Kristin Johnson

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