Department of Chemistry

College of Letters & Science
Kaur H, Groubert B, Paulson JC, McMillan S, Hoskins AA. Impact of cancer-associated mutations in Hsh155/SF3b1 HEAT repeats 9-12 on pre-mRNA splicing in Saccharomyces cerevisiae. Plos One. 2020;15.
Kaur H, Jamalidinan F, Condon SGF, Senes A, Hoskins AA. Analysis of spliceosome dynamics by maximum likelihood fitting of dwell time distributions. Methods. 2019;153:13-21.
Hansen SR, Nikolai BJ, Spreackert PJ, Carrocci TJ, Hoskins AA. Chemical Inhibition of Pre-mRNA Splicing in Living Saccharomyces cerevisiae. Cell Chemical Biology. 2019;26:443-448.
Beier DH, Carrocci TJ, van der Feltz C, Tretbar US, Paulson JC, Grabowski N, et al.. Dynamics of the DEAD-box ATPase Prp5 RecA-like domains provide a conformational switch during spliceosome assembly. Nucleic Acids Research. 2019;47:10842-10851.
Condon SGF, Deena-Al M, Armstrong CR, Diaz-Vazquez G, Craven SJ, LaPointe LM, et al.. The FtsLB subcomplex of the bacterial divisome is a tetramer with an uninterrupted FtsL helix linking the transmembrane and periplasmic regions. Journal of Biological Chemistry. 2018;293:1623-1641.
Carrocci TJ, Paulson JC, Hoskins AA. Functional analysis of Hsh155/SF3b1 interactions with the U2 snRNA/branch site duplex. RNA. 2018;24:1028-1040.
van der Feltz C, DeHaven AC, Hoskins AA. Stress-induced Pseudouridylation Alters the Structural Equilibrium of Yeast U2 snRNA Stem II. Journal of Molecular Biology. 2018;430:524-536.
Carrocci TJ, Lohe L, Ashton MJ, Hobartner C, Hoskins AA. Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides. Chemical Communications. 2017;53:11992-11995.
Larson JD, Hoskins AA. Dynamics and consequences of spliceosome E complex formation. eLife. 2017;6.
van der Feltz C, Hoskins AA. Methodologies for studying the spliceosome's RNA dynamics with single-molecule FRET. Methods. 2017;125:45-54.
Panchapakesan SSS, Ferguson ML, Hayden EJ, Chen X, Hoskins AA, Unrau PJ. Ribonucleoprotein purification and characterization using RNA Mango. RNA. 2017;23:1592-1599.
Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA. SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast. Nucleic Acids Research. 2017;45:4837-4852.
Didychuk AL, Montemayor EJ, Carrocci TJ, DeLaitsch AT, Lucarelli SE, Westler WM, et al.. Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities. Nature Communications. 2017;8.
Hansen SR, Rodgers ML, Hoskins AA. Fluorescent Labeling of Proteins in Whole Cell Extracts for Single-Molecule Imaging. In: Spies M, Chemla YR. 2016. pp. 83-104.
Rodgers ML, Tretbar US, Dehaven A, Alwan AA, Luo G, Mast HM, et al.. Conformational dynamics of stem II of the U2 snRNA. RNA. 2016;22:225-236.
Xu J, Carrocci TJ, Hoskins AA. Evolution and characterization of a benzylguanine-binding RNA aptamer. Chemical Communications. 2016;52:549-552.
DeHaven AC, Norden IS, Hoskins AA. Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy. Wiley Interdisciplinary Reviews-Rna. 2016;7:683-701.
Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA. A multi-step model for facilitated unwinding of the yeast U4/U6 RNA duplex. Nucleic Acids Research. 2016;44:10912-10928.
Hoskins AA, Rodgers ML, Friedman LJ, Gelles J, Moore MJ. Single molecule analysis reveals reversible and irreversible steps during spliceosome activation. eLife. 2016;5.
Cornilescu G, Didychuk AL, Rodgers ML, Michael LA, Burke JE, Montemayor EJ, et al.. Structural Analysis of Multi-Helical RNAs by NMR-SAXS/WAXS: Application to the U4/U6 di-snRNA. Journal of Molecular Biology. 2016;428:777-789.
Rodgers ML, Paulson J, Hoskins AA. Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull. RNA. 2015;21:1031-1041.
Kimble J, Bement WM, Chang Q, Cox BL, Drinkwater NR, Gourse RL, et al.. Strategies from UW-Madison for rescuing biomedical research in the US. eLife. 2015;4.
Anderson EG, Hoskins AA. Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis. In: Hertel KJ. 2014. pp. 217-241.
Trang VH, Rodgers ML, Boyle KJ, Hoskins AA, Strieter ER. Chemoenzymatic Synthesis of Bifunctional Polyubiquitin Substrates for Monitoring Ubiquitin Chain Remodeling. Chembiochem. 2014;15:1563-1568.
Wang TY, Friedman LJ, Gelles J, Min W, Hoskins AA, Cornish VW. The Covalent Trimethoprim Chemical Tag Facilitates Single Molecule Imaging with Organic Fluorophores. Biophysical Journal. 2014;106:272-278.
Larson J, Kirk M, Drier EA, O'Brien W, MacKay JF, Friedman LJ, et al.. Design and construction of a multiwavelength, micromirror total internal reflectance fluorescence microscope. Nature Protocols. 2014;9:2317-2328.
Carrocci TJ, Hoskins AA. Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores. Analyst. 2014;139:44-47.
Larson JD, Rodgers ML, Hoskins AA. Visualizing cellular machines with colocalization single molecule microscopy. Chemical Society Reviews. 2014;43:1189-1200.
Shcherbakova I, Hoskins AA, Friedman LJ, Serebrov V, Correa IR, Xu MQ, et al.. Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy. Cell Reports. 2013;5:151-165.
Crawford DJ, Hoskins AA, Friedman LJ, Gelles J, Moore MJ. Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5 ' splice site and branch site. Proceedings of the National Academy of Sciences of the United States of America. 2013;110:6783-6788.
Hoskins AA, Moore MJ. The spliceosome: a flexible, reversible macromolecular machine. Trends in Biochemical Sciences. 2012;37:179-188.
Hoskins AA, Gelles J, Moore MJ. New insights into the spliceosome by single molecule fluorescence microscopy. Current Opinion in Chemical Biology. 2011;15:864-870.
Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, Wombacher R, et al.. Ordered and Dynamic Assembly of Single Spliceosomes. Science. 2011;331:1289-1295.
Morar M, Hoskins AA, Stubbe J, Ealick SE. Formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima: Structural insights into complex formation. Biochemistry. 2008;47:7816-7830.
Crawford DJ, Hoskins AA, Friedman LJ, Gelles J, Moore MJ. Visualizing the splicing of single pre-mRNA molecules in whole cell extract. RNA. 2008;14:170-179.
Hoskins AA, Morar M, Kappock TJ, , Zaugg JB, Barder TE, et al.. N-5-CAIR mutase: Role of a CO2 binding site and substrate movement in catalysis. Biochemistry. 2007;46:2842-2855.
Morar M, Anand R, Hoskins AA, Stubbe J, Ealick SE. Complexed structures of formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima describe a novel ATP binding protein superfamily. Biochemistry. 2006;45:14880-14895.
Anand R, Hoskins AA, Stubbe J, Ealick SE. Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography. Biochemistry. 2004;43:10328-10342.
Hoskins AA, Anand R, Ealick SE, Stubbe J. The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: Metabolite-mediated complex formation. Biochemistry. 2004;43:10314-10327.
Anand R, Hoskins AA, Bennett EM, Sintchak MD, Stubbe J, Ealick SE. A model for the Bacillus subtilis formylglycinamide ribonucleotide amidotransferase multiprotein complex. Biochemistry. 2004;43:10343-10352.
Ilin S, Hoskins AA, Schwalbe H, Wohnert J. NMR assignment of the full-length ribosomal protein L11 from Thermotoga maritima. Journal of Biomolecular NMR. 2003;25:163-164.