Department of Chemistry

College of Letters & Science
Joshua J. Coon

Email address: jcoon@chem.wisc.edu

Room Number: 
4426 Biotech
Telephone Number: 
Group Affiliation: 
Coon Group
Position Name: 
Chemical Biology

B.S., Central Michigan University                
Ph.D. 2002, University of Florida
Postdoctoral Fellow at University of Virginia

Also: Professor of Biomolecular Chemistry
Director, NIGMS National Center for Quantitative Biology of Complex Systems

jcoon's picture

Research Description

Research Emphasis: Bioanalytical Chemistry, Mass Spectrometry & Proteomics

My research group has the overarching goal of catalyzing evolution in the rapidly developing field of proteomics and to use these technologies to address fundamental problems in developmental biology. With emphasis on ion chemistry and instrumentation, we seek to develop and apply new enabling mass spectrometry-based (MS) proteomic technologies. These cutting-edge tools allow us to examine, with unprecedented chemical detail and sensitivity, the molecular events that commit human embryonic stem cells (hES cells) to exit the pluripotent state. Here we are focused on both intracellular signaling and the epigenetic regulation of pluripotency. For the former we ask which branches of the FGF signaling pathway are active in hES cells and which proteins/networks are phosphorylated upon differentiation. Epigenetics is believed to play a critical role in the establishment and maintenance of pluripotency; thus, we have also aimed our new technologies at interpreting the epigenetic codes and monitoring how these messages change during hES cell differentiation.

Active projects
Research projects in the Coon lab include: (1) instrumentation development, (2) data analysis software design, (3) fundamental ion chemistry studies, and (4) biological applications of the technology. Biological applications include global identification of protein post-translational modification (specifically phosphorylation), quantitative analysis of protein phosphorylation (i.e., comparative analysis of two cellular states), and cancer biomarker discovery.

Awards and Honors

WARF Romnes Faculty Fellowship, University of Wisconsin-Madison 2014
Biemann Medal, American Society for Mass Spectrometry 2012
Arthur F. Findeis Award for Achievements by a Young Analytical Scientist, American Chemical Society 2011
Philip R. Certain Dean's Distinguished Faculty Award 2010
Kellett Mid-Career Award 2020

Selected Publications

Rahimi A, Ulbrich A, Coon JJ, Stahl SS. Formic-acid-induced depolymerization of oxidized lignin to aromatics. Nature. 2014;515:249-52.
Steinert ND, Potts GK, Wilson GM, Klamen AM, Lin KH, Hermanson JB, et al. Mapping of the contraction-induced phosphoproteome identifies TRIM28 as a significant regulator of skeletal muscle size and function. Cell Reports. 2021;34.
Reddy TR, Overmyer KA, Coon JJ, Drake M, Horiba T, Rankin SA. Metabolomic Biomarkers Differentiate Soy Sauce Freshness under Conditions of Accelerated Storage. Journal of Food Quality. 2021;2021.
Lin KH, Wilson GM, Blanco R, Steinert ND, Zhu WYG, Coon JJ, et al. A deep analysis of the proteomic and phosphoproteomic alterations that occur in skeletal muscle after the onset of immobilization. Journal of Physiology-London. 2021;.
He YC, Rashan EH, Linke V, Shishkova E, Hebert AS, Jochem A, et al. Multi-Omic Single-Shot Technology for Integrated Proteome and Lipidome Analysis. Analytical Chemistry. 2021;93:4217-4222.