UNIVERISTY of WISCONSIN-MADISON

Department of Chemistry

College of Letters & Science
Joseph Kim

Email address: jykim35@wisc.edu

Room Number: 
461 HFD Biochemistry Laboratories
Position Name: 
Graduate Student
Path: 
Physical
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Education

H.B.S., summa cum laude, 2018, Oregon State University (Biochemistry & Biophysics)

Research Description

In Elizabeth Wright's lab at the Department of Biochemistry, we are interested in using cryo-electron tomography (cryo-ET) to examine the structure and organization of biological macromolecular assemblies at nanometer to sub-nanometer resolution within the native cellular context. This is done by freezing biological samples at temperatures of around -170 degrees celsius to preserve the sample in its hydrated native state to be examined under a transmission electron microscope (TEM). My interests lie in examining such assemblies in neurobiology, which has recently caught the interest of many investigators within the cryo-EM community, especially in the context of molecular/cellular function and neurodegeneration. To aid in this endeavor, I am combining cryo-ET with cryogenic fluorescent light microscopy (cryo-FLM) to fluorescently label specific targets and focused ion beam with a scanning electron microscope (FIB-SEM) to thin biological samples in cryogenic conditions to be examined under the TEM.