Paper Chromatography

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Description
Preparing the Developing Chamber
Preparing the Stationary Phase
Loading the Sample on the Paper
Developing the Chromatogram
Identifying the Spots
Interpreting the Chromatogram
Additional Topics
Self Check Exercises
Related Modules
Gas Chromatography
Thin-Layer Chromatography
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Self Check Exercises

 

IMAGE. A separated sample.


IMAGE. A drawing of a chromatogram.

check A student removed the chromatogram in the upper image from the development chamber and allowed the solvent to dry. What did he forget to do? Answer

The student forgot to trace the solvent front before allowing the solvent to dry on the chromatogram. No Rf values can be calculated from this chromatogram.

check The chromatogram in the lower image was spotted with three samples: Sample 1, Sample 2, and Sample 3. What conclusions can be drawn about the components of Sample 3? Answer

Sample 3 is composed of at least two different compounds, one of which is probably the same as Sample 1. Sample 3 does not contain any of Sample 2, but does contain another component which is not Sample 1 or Sample 2.