Centrifuge

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Description
Safety
Loading a Centrifuge
Centrifuging a Sample
Unloading a Centrifuge
Additional Topics
Removing the Supernatant
Rinsing the Solid
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Loading a Centrifuge

 

Video. Use a counterweight

The mixture should be transferred to a clean centrifuge tube (a test tubecan be used). Choose a centrifuge tube that fits well in the sample slots. The tube should not extend above the top of the centrifuge. The top of the tube should not rest within the sample slot. If the tube is too short, then you will have difficulty in removing it from the centrifuge.

check Why should the tube not extend above the centrifuge? Answer

The centrifuge will spin at a very high rate, thus a test tube that is extending outside of the centrifuge is a hazardous situation.

Prepare a second centrifuge tube to counterbalance the sample. The counterbalance tube should weigh approximately the same as the sample. Usually, this second tube is prepared by adding distilled water to a centrifuge tube until the volume is equal to the volume of the sample. Then, the counterweight tube is placed in the centrifuge in the slot directly opposite of the sample.

check Why use a counterweight? Answer

Video. An unbalanced centrifuge.

A counterweight is used to balance the centrifuge while it is operating. An unbalanced centrifuge will vibrate and may move across the lab bench.