Scanning Spectrophotometer

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Description
Preparing the Cuvets
Preparing the Reference
Solution
Zeroing the Spectrometer
Recording a Spectrum
Additional Topics
Other Types of Spectrometers
Related Modules
Spectrometer, Spectronic 20™
Thin-Layer Chromatog-
raphy
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Description

 

IMAGE. The Perkin Elmer Lambda 11™
Single Beam UV/Vis Spectrophotometer

A scanning ultraviolet/visible (UV/Vis) spectrophotometer operates on the same principles as a Spectronic 20™. They both can be used for qualitative and quantitative analysis.

A scanning spectrophotometer's main advantage over the Spectronic 20™ is that the scanning spectrophotometer can rapidly scan a range of wavelengths and record absorbances at each wavelength. In this module, the Perkin Elmer Lambda 11™, a single beam spectrophotometer, is described but similar principles apply to all UV/Vis spectrophotometers. For specific operating instructions, refer to the operator's manual or other instructions. Double beam and diode array spectrophotometers are also commonly used to obtain spectra.