The developing chamber in the Self Check image 1 has been standing for a few minutes. Why is it not ready to be used in a TLC experiment?
Answer
Although the jar has been standing for a few minutes, the atmosphere inside it could not have become saturated with solvent since it was not tightly covered. Any solvent vapor would escape the jar into the room.
What is wrong with the plate shown in Self Check image 2?
Answer
The samples were spotted on the plate too closely together. During the development of the plate, the spots will run together, causing inconclusive experimental results.
Predict what will happen when the plate in Self Check image 3 is developed in the chamber.
Answer
The samples were spotted too low on the plate, and the chamber is too full with the developing solvent. Therefore, when the plate is placed into the chamber, the spots will bleed off of
the plate and into the solvent. The plate cannot be developed.
The developing chamber in the Self Check image 1 has been standing for a few minutes. Why is it not ready to be used in a TLC experiment?