IMAGE. Self Check image 1.
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IMAGE. Self Check image 2.
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A student removes his TLC plate from the chamber after the solvent reaches
the etched pencil mark, but he cannot see any spots on the plate. What technique would you suggest to help him identify the spots?
Answer
If the plate is held under an ultraviolet lamp, some of the spots may become visible.
Calculate the Rf values for the spots on the TLC in Self Check image 1.
Answer
Measure the distances from the starting point to the solvent front and from the starting point to the center of each spot, and calculate the Rf values: 0.28, 0.83, and 0.59 from left to right.<
The chromatogram in the lower image was spotted with three samples: Sample 1, Sample 2, and Sample 3. What conclusions can be drawn about the components of Sample 3?
Answer
Sample 3 is composed of at least two different compounds, one of which is probably the same as Sample 1. Sample 3 does not contain any of Sample 2, but does contain another component which is not Sample 1 or Sample 2. |
